- “You misunderstand. This isn't about revenge! It's about feeding my brood! And fatboy her leaves plenty for seconds.”
- —The Queen to Jay
- “Guess what, chubbo. I'm larger!”
- —The Queen to Emperor Worm before taking off her disguise
The Bug Queen (also referred to as Queenie by her children) is the mother and ruling monarch of the entire alien Bug race.
She sends Edwin to go get whoever killed Edgar the Bug during his quest for the Arquilian Galaxy. Edwin finds Elle after a short quest (Elle gave the final shot to Edgar), and kidnaps her. Then Jay, Kay, and the Worms find Elle and Jay, Kay, and Elle are captured. Luckily, the Worms revive some betrayed bugs, who kill Edwin before they are taken to the Queen. However, the Queen and some Bugs would return. She only craves sugar.
Later, many other Bugs come to Earth. They protect the Queen who is disguised as a small girl, and came to Earth to lay her eggs. However, she encounters the Emperor Worm, when she reveals her true form. After a quest, Kay, Jay, and Elle are able to save the Worm, and destroy the Queen's eggs and larvae. The Queen then falls into a pool with a neon sign which electrocutes her. Finally, the agents freeze the Queen.
- At the beginning of MiB 1, James Edwards (later J), shot a cutout of a small girl (Tiffany). When the Bug Queen came to earth with a small girl disguise, the girl is the same as Tiffany.
- While the other bugs such as Edgar the Bug and the Queen's two bodyguards struggled hard to fit in with their human suits, the Queen fit in with hers almost perfectly and talked in a more normal human speech pattern until she shed her disguise.